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زیست شناسی::
جهش یافته
Many S. cerevisiae selection schemes rely on mutant host strains that require a particular amino acid (histidine, tryptophan, or leucine) or nucleotide (uracil) for growth.
For example, when a plasmid vector with a wildtype LEU2 gene is transformed into a mutant leu2 host cell and plated onto medium that lacks leucine, only cells that carry the plasmid will grow.
(A) A single recombination (dashed line) between the HIS4 gene of an intact circular plasmid and a chromosome his4 mutant gene re sults in the integration of the entire vector, including the gene of interest (GOI) with the AOX1 promoter in the 5?
The inserted DNA is flanked by recombined mutant his4 and functional HIS4 genes.
In addition to isolating natural genes that encode proteins with useful properties, directed or random mutagenesis and selection schemes can be used to create a mutant form of a gene that encodes a protein with the desired properties.
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