داستان آبیدیک

nucleotide


فارسی

1 زیست شناسی:: نوکلئوتید

Many S. cerevisiae selection schemes rely on mutant host strains that require a particular amino acid (histidine, tryptophan, or leucine) or nucleotide (uracil) for growth. To eliminate the need to use plaque assays to identify and purify recom­ binant viruses, several methods have been developed that introduce the target gene into the baculovirus genome at a specific nucleotide sequence by recombination, either in an intermediate bacterial host, such as E. coli, or in vitro outside of a host cell. Initiation of translation in higher eukaryotic organisms depends on a specific sequence of nucleotides surrounding the start (AUG) codon in the mRNA called the Kozak sequence (e.g. In directed mutagenesis, a specific amino acid is tar­ geted for change by substituting nucleotides in the corresponding coding sequence in the gene. A large number of mu­ tant proteins, each with a different amino acid change, are generated by randomly altering individual nucleotides within a structural gene, and then tested for the desired properties.

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