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نوکلئوتید
Many S. cerevisiae selection schemes rely on mutant host strains that require a particular amino acid (histidine, tryptophan, or leucine) or nucleotide (uracil) for growth.
To eliminate the need to use plaque assays to identify and purify recom binant viruses, several methods have been developed that introduce the target gene into the baculovirus genome at a specific nucleotide sequence by recombination, either in an intermediate bacterial host, such as E. coli, or in vitro outside of a host cell.
Initiation of translation in higher eukaryotic organisms depends on a specific sequence of nucleotides surrounding the start (AUG) codon in the mRNA called the Kozak sequence (e.g.
In directed mutagenesis, a specific amino acid is tar geted for change by substituting nucleotides in the corresponding coding sequence in the gene.
A large number of mu tant proteins, each with a different amino acid change, are generated by randomly altering individual nucleotides within a structural gene, and then tested for the desired properties.
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