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زیست شناسی::
پروکاریوت
The basic requirements for expression of a target protein in a eukaryotic host are similar to those required in prokaryotes.
Because recombinant DNA procedures are technically more difficult to carry out with eukaryotic cells, they are typically carried out in prokaryotic cells before transferring the vectors to the eukaryotic host for protein expression.
They have growth advantages similar to those of prokaryotes, such as rapid growth in lowcost medium; generally do not require growth factors to be added to the growth medium; can correctly process eukaryotic proteins; and can secrete large amounts of heterolo gous proteins.
In prokaryotes, a promoter sequence is necessary for the ini tiation of transcription at the correct nucleotide site, and a terminator sequence at the end of the gene is essential for the cessation of transcription.
Although many heterologous proteins have been suc cessfully synthesized in prokaryotic host cells, some proteins require eukaryotespecific posttranslational modifications, such as glycosylation, to be functional.
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