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زیست شناسی::
سابتیلیزین
Subtilisins are a group of nonspecific serine proteases that are secreted into growth medium by Gram positive bacteria and are widely used as bio degradable cleaning agents in laundry detergents.
All subtilisins bind tightly (affinity constant [Ka] = ~107 M) to one or more molecules of calcium per molecule of enzyme where calcium binding stabilizes the enzyme.
Un fortunately, since subtilisins are used in industrial settings where there are a large number of chelating agents that can bind to and effectively remove calcium, these enzymes are rapidly inactivated under these conditions.
circumvent this problem, it is necessary first to abolish completely the abil ity of a subtilisin to bind calcium and then to attempt to increase the stability of this modified enzyme in the absence of bound calcium.
The starting point for the development of a modified subtilisin was an iso lated subtilisin gene from Bacillus amyloliquefaciens.
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