neomycin

1 زیست شناسی:: نئومایسین

For example, the bacterial neo gene, which encodes neomycin phosphotrans­ ferase, is often used to select transfected mammalian cells. In eukaryotic cells, G­418 (Geneticin), which is phosphorylated and thereby inactivated by neomycin phosphotransferase, replaces neomycin as the selective agent be­ cause neomycin is not an effective inhibitor of eukaryotic protein synthesis. Selective agent Action of selective agent Marker gene Action of marker gene protein Xyl-A Damages DNA Adenine deaminase (ada) Deaminates Xyl-A Blasticidin S Inhibits protein synthesis Blasticidin S deaminases (Bsr, BSD) Deaminates blasticidin S Bleomycin Breaks DNA strands Bleomycin-binding protein (Ble) Binds to bleomycin G-418 (Geneticin) Inhibits protein synthesis Neomycin phosphotransferase (neo) Phosphorylates G-418 Histidinol Produces cytotoxic efects Histidinol dehydrogenase (hisD) Oxidizes histidinol to histidine Hygromycin B Inhibits protein synthesis Hygromycin B phosphotransferase (Hph) Phosphorylates hygromycin B MSX Inhibits glutamine synthesis Glutamine synthetase (GS) Cells that produce excess glutamine synthetase survive MTX Inhibits DNA synthesis Dihydrofolate reductase (dhfr) Cells that produce excess dihydrofolate reductase survive PALA Inhibits purine synthesis Cytosine deaminase (codA) Lowers cytosine levels in the medium by converting cytosine to uracil Puromycin Inhibits protein synthesis Puromycin N-acetyltransferase (Pac) Acetylates puromycin MSX, methionine sulfoximine; MTX, methotrexate; PALA, N­(phosphoacetyl)­l­aspartate; Xyl­A, 9­ک­d­xylofuranosyl adenine.

واژگان شبکه مترجمین ایران