dehydrogenase

1 زیست شناسی:: دهیدروژناز

Promoter Expression conditions Status Acid phosphatase (PH05) Phosphate-deficient medium Inducible medium Alcohol dehydrogenase I (ADHI) 2-5% Glucose Constitutive Alcohol dehydrogenase II (ADHII) 0.1-0.2% Glucose Inducible Cytochrome c1 (CYC1) Glucose Repressible Gal-1-P Glc-1-P uridyltransferase Galactose Inducible Galactokinase (GAL1) Galactose Inducible Glyceraldehyde-3-phosphate dehydrogenase (GAPD, GAPDH) 2-5% Glucose Constitutive Metallothionein (CUP1) 0.03-0.1 mM Copper Inducible Phosphoglycerate kinase (PGK) 2-5% Glucose Constitutive Triosephosphate isomerase (TPI) 2-5% Glucose Constitutive UDP-galactose epimerase (GAL10) Galactose Inducible To test this hypothesis, the yeast protein disulfide isomerase gene was cloned between the constitutive glyceraldehyde phosphate dehydrogenase promoter The HIS4 gene encodes a functional histidinol dehydrogenase of the histidine biosynthesis pathway. Selective agent Action of selective agent Marker gene Action of marker gene protein Xyl-A Damages DNA Adenine deaminase (ada) Deaminates Xyl-A Blasticidin S Inhibits protein synthesis Blasticidin S deaminases (Bsr, BSD) Deaminates blasticidin S Bleomycin Breaks DNA strands Bleomycin-binding protein (Ble) Binds to bleomycin G-418 (Geneticin) Inhibits protein synthesis Neomycin phosphotransferase (neo) Phosphorylates G-418 Histidinol Produces cytotoxic efects Histidinol dehydrogenase (hisD) Oxidizes histidinol to histidine Hygromycin B Inhibits protein synthesis Hygromycin B phosphotransferase (Hph) Phosphorylates hygromycin B MSX Inhibits glutamine synthesis Glutamine synthetase (GS) Cells that produce excess glutamine synthetase survive MTX Inhibits DNA synthesis Dihydrofolate reductase (dhfr) Cells that produce excess dihydrofolate reductase survive PALA Inhibits purine synthesis Cytosine deaminase (codA) Lowers cytosine levels in the medium by converting cytosine to uracil Puromycin Inhibits protein synthesis Puromycin N-acetyltransferase (Pac) Acetylates puromycin MSX, methionine sulfoximine; MTX, methotrexate; PALA, N­(phosphoacetyl)­l­aspartate; Xyl­A, 9­ک­d­xylofuranosyl adenine. Under these conditions, pyruvate, an intermediate compound produced during the metabolism of glucose, is converted to lactate by lactate dehydrogenase rather than entering into the tricarboxylic acid cycle, where it is further oxidized through the activity of pyruvate carboxylase (Fig. 3.44).

واژگان شبکه مترجمین ایران