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عمومی::
تفکیک
In some cases, the humanized antibody or fragment or derivative thereof has an equilibrium dissociation constant of 1.86 � 10-8 M or less for the tau protein or tau peptide.
[0098] The term "Kassoc" or "Ka", as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction, whereas the term "Kdis" or "Kd," as used herein, is intended to refer to the dissociation rate of a particular antibody-antigen interaction.
The term "KD", as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e.
, equilibrium dissociation constant (KD).
A solution of each antibody, prepared with a HEPES buffer (pH 7.2) as a specific binding reaction solution, was applied to the sensor chip surface at concentrations ranging from 0.2 to 1 nM (around the estimated binding dissociation constant [KD]) for 150 seconds (uniform in the same evaluation) for a reaction, and the binding kinetics were measured.
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