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زیست شناسی::
P. pastoris
Pichi pastoris is a methylotrophic yeast that is able to utilize methanol as a source of energy and carbon.
With these natural characteristics as a starting point, a P. pastoris strain was extensively engineered with the aim of developing a "humanized" strain that glycosylates proteins in a manner identical to that of human cells.
To create a "humanized" strain, the enzyme responsible for addition of the a1,6 mannose was first elimi nated from P. pastoris to prevent hypermannosylation.
Figure 3.30 Differential processing of glycoproteins in P. pastoris, humans, and "humanized" P. pastoris.
Initial additions of sugar residues to glycoproteins in the endoplasmic reticulum are similar in human and P. pastoris cells (A).
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